Genetic polymorphisms of four STR loci on chromosome X and their forensic applications in a Chinese Han population / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 268-271, 2005.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-321109
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To add DXS7133, GATA198A10, DXS9896 and DXS6797 to the panel of forensically validated X chromosome markers, and apply the multiplex amplification system to a population study and forensic analysis on the Hans of Chengdu.</p><p><b>METHODS</b>The PCR products were detected by the polyacrylamide gel electrophoresis and silver staining method. Hardy-Weinberg equilibrium of females was tested and every forensically interested value was calculated.</p><p><b>RESULTS</b>Sequencing revealed that their common sequence motifs were tetranucleotide repeats. Population genetic data were obtained by analyzing 120 unrelated females and 100 males from Chengdu Han ethnic group. In this population, DXS7133, GATA198A10, DXS9896 and DXS6797 exhibited 6, 6, 11, 8 distinguishable alleles respectively. Chi-square test demonstrated that genotype frequencies in females did not depart from Hardy-Weinberg equilibrium. Power of discrimination for female samples for the four loci were 0.7962, 0.8021, 0.9675, and 0.9444. The parentage testing in 32 cases revealed a typical X-linked inheritance and no mutations.</p><p><b>CONCLUSION</b>DXS7133, GATA198A10, DXS9896 and DXS6797, which are highly polymorphic in Chengdu Han population, are appropriate for individual identification and paternity testing involving a female child.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Distribuição de Qui-Quadrado
/
China
/
Reação em Cadeia da Polimerase
/
Repetições de Microssatélites
/
Cromossomos Humanos X
/
Povo Asiático
/
Frequência do Gene
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Genética
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Genótipo
Limite:
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2005
Tipo de documento:
Artigo