Granulocyte-macrophage colony-stimulating factor and interleukin 4 induce the malignant transformation of the bone marrow-derived human adult mesenchymal stem cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 729-733, 2011.
Article
em En
| WPRIM
| ID: wpr-321429
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The purpose of the study was to examine the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) on the bone-marrow-derived human adult mesenchymal stem cells (hMSCs).</p><p><b>METHODS</b>The hMSCs were isolated and cultured with GM-CSF and IL-4 for a period of one month. A single colony of transformed cells was then isolated and their phenotype was characterized by morphology, surface marker expression, and in vivo tumorigenesis.</p><p><b>RESULTS</b>After one month culture, the transformed mesenchymal cells exhibited the morphology and phenotype similar to those of tumor cells, and also caused multiple fast growing lung deposits when it was injected into immunodeficient mice.</p><p><b>CONCLUSION</b>Cytokines-driven malignant transformation of hMSCs may be a useful model for studying signaling pathways initiating malignant transformation of hMSC.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Células da Medula Óssea
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Imuno-Histoquímica
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Transformação Celular Neoplásica
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Células Cultivadas
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Fator Estimulador de Colônias de Granulócitos e Macrófagos
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Interleucina-4
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Biologia Celular
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Células-Tronco Mesenquimais
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Citometria de Fluxo
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2011
Tipo de documento:
Article