Serine residues at position 63-84 are important for CITED1 nuclear translocation and osteoblast differentiation / 南方医科大学学报
Journal of Southern Medical University
; (12): 326-331, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-322052
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the role of serine residues at position 63-84 of CITED1 in the nuclear translocation of CITED1 and osteoblast differentiation.</p><p><b>METHODS</b>We engineered all the 9 phosphorylated serine residues of CITED1 with a serine-to-alanine mutation at position 63-84. MC3T3E1 cells transfected with pCDNA3-CFP-CITED1 63-84 (9S>A), pCDNA3-CFP-CITED1, and vehicle plasmid were examined with confocal laser scanning microscopy before and after treatment with 100 nmol/L parathyroid hormone [PTH(1-34)] to observe the changes in the intracellular localization of CITED1. The transfected cells were induced for osteoblastic differentiation with mineralized solution in the absence or presence of 10 nmol/L PTH(1-34), and the changes in ALP activity and Ca(2+) concentration were measured; RT-PCR was used to detect the changes in ALP2, RUNX2, and OC gene expressions after the treatments.</p><p><b>RESULTS</b>s PTH(1-34) promoted the nuclear translocation of CITED1 in MC3T3-E1 cells. The (63-84) 9S>A mutation of CITED1 obviously suppressed its translocation and increased ALP activity and Ca(2+) levels in the cells, which led to enhanced mineralization in the cells with also increased expressions of ALP2, RUNX2, and OC.</p><p><b>CONCLUSION</b>The serine residues at position 63-84 of CITED1 play a vital role in the nuclear translocation of CITED1 and osteoblast differentiation.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Plasmídeos
/
Serina
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Proteínas Nucleares
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Diferenciação Celular
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Transativadores
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Linhagem Celular
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Núcleo Celular
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Transporte Ativo do Núcleo Celular
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Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo