Study on kinetics of two-stage cultivation of protocorm-like bodies from Dendrobium huoshanense for cell growth and synthesis of polysaccharides / 生物工程学报
Chinese Journal of Biotechnology
; (12): 79-84, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-325415
Biblioteca responsável:
WPRO
ABSTRACT
Phosphate is an effective factor in the regulation of cell growth and accumulation of polysaccharides. The method for higher polysaccharide production has been obtained through feeding cultivation. The two-stage cultivation was proposed according to the characteristic of cell growth and the accumulation of polysaccharides in the suspension cultures of protocorm-like bodies from Dendrobium huoshanense. A kinetic model was developed to describe the cultivation process. The results indicate that the production of biomass and polysaccharides increases from 28.7g DW/L and 1.86g/L to 44.2g DW/L and 5.22g/L, respectively, and the content of polysaccharides increases from 6.4% to 11.9% . With the evaluated model parameters, the model appears to provide a description for the cultivation process.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polissacarídeos
/
Fatores de Tempo
/
Algoritmos
/
Cinética
/
Células Cultivadas
/
Caules de Planta
/
Biomassa
/
Técnicas de Cultura de Células
/
Biologia Celular
/
Dendrobium
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Artigo