Expression of porcine beta-defensin 1 gene in Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1036-1039, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-325429
Biblioteca responsável:
WPRO
ABSTRACT
PBD-1 is an antibacterial peptide that plays an important role in defence system of porcine. To produce PBD-1 with bioactivity in Pichia pastoris, according to published amino acid sequence of porcine beta-defensin 1(PBD-1) and the partiality codon of yeast, the PBD-1 gene was synthesized by PCR and cloned into pPIC9K to construct the recombinant expression vector pPIC9K-PBD-1, the obtained recombinant plasmid was linearized by Sal I, and then transformed into SMD1168 by electroporation. Under the control of the promoter AOX1, an approximately 4.5 kD PBD-1 peptide was expressed. Antibacterial activity assay shows that the PBD-1 has the antibacterial activity on Staphylococcus aureus. This is the first secreted expression of porcine beta-defensin 1 gene in Pichia pastoris.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Pichia
/
Staphylococcus aureus
/
Suínos
/
Engenharia de Proteínas
/
Expressão Gênica
/
Clonagem Molecular
/
Beta-Defensinas
/
Vetores Genéticos
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo