Phytochemical Analysis of the Phenolic Fat-Suppressing Substances in the Leaves of Lactuca raddeana in 3T3-L1 Adipocytes
Natural Product Sciences
; : 42-48, 2015.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-32656
Biblioteca responsável:
WPRO
ABSTRACT
Lactuca raddeana (Compositae) is used to treat obesity and complications due to diabetes. The five phenolic compounds including chlorogenic acid, chicoric acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, luteolin were qualitatively identified by LC-ESI-MS analysis. The contents were quantitatively determined by HPLC, under the condition of a Capcell Pak C18 column (5 microm, 250 mm x 4.6 mm i.d.) and a gradient elution of 0.05% trifluoroacetic acid (TFA) and 0.05% TFA in MeOH-H2O (60 40). The contents of chicoric acid (100.99 mg/g extract) and luteolin 7-O-glucoside (101. 69 mg/g extract) were high, while those of other three phenolic substances were very low. The 3T3-L1 adipocyte cells treated with chicoric acid and luteolin 7-O-glucuronide significantly suppressed the accumulation of fat, suggesting they are effective against obesity. Since high level of peroxynitrite (ONOO) causes cardiovascular disease in obese patients, its scavenging activity was also studied.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácido Trifluoracético
/
Doenças Cardiovasculares
/
Ácido Clorogênico
/
Cromatografia Líquida de Alta Pressão
/
Adipócitos
/
Asteraceae
/
Fenol
/
Ácido Peroxinitroso
/
Luteolina
/
Obesidade
Limite:
Humanos
Idioma:
Inglês
Revista:
Natural Product Sciences
Ano de publicação:
2015
Tipo de documento:
Artigo