Effect of Pingtang recipe containing drug-serum on INS-1 pancreatic beta cells lipoapoptosis / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 978-981, 2010.
Article
em Zh
| WPRIM
| ID: wpr-327500
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect and mechanism of Pingtang Recipe containing drug-serum (DS-PTR) in improving INS-1 beta pancreatic cells lipoapoptosis.</p><p><b>METHODS</b>Experimental INS-1 beta cells were divided into 5 groups (6 pools for each group), namely, the blank control group treated with rat's serum (C), the other 4 model groups induced into lipoapoptosis by palmitic acid and treated respectively by rat's serum (M), high, middle and low dose DS-PTR (DSh, DSm and DSI). Cell apoptosis was detected by TUNEL staining; Caspase-3 activity of cells was measured by chemiluminescence method; intracellular production of reactive oxygen species (ROS) was detected by DCHF-DA incorporation, and expressions of uncoupling protein-2 (UCP-2) was determined by RT-PCR.</p><p><b>RESULTS</b>INS-1 beta cell apoptosis in Group M was significantly higher than that in Group C (P < 0.01), while that showed a decreased trend in the three DS-PTR treated groups. Caspase-3 activity was enhanced in Group M, it decreased significantly in Group DSm (P < 0.05). The over-produced ROS in cells after modeling was inhibited in Groups DSm and DSI (P < 0.05), meantime, expression of UCP-2 excited by PA (2.244 +/- 0.421) was reduced significantly in Group DSI and Group DSm to 1.286 +/- 0.373 (P < 0.01) and 1.627 +/- 0.348 (P < 0.05) respectively.</p><p><b>CONCLUSION</b>DS-PTR shows a protective effect on INS-1 beta pancrentic cells against lipoapoptosis, which is possibly play its mechanism through regulating ROS and UCP-2.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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RNA Mensageiro
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Medicamentos de Ervas Chinesas
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Linhagem Celular
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Espécies Reativas de Oxigênio
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Ratos Sprague-Dawley
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Apoptose
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Biologia Celular
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Proteínas Mitocondriais
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Soro
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2010
Tipo de documento:
Article