Soluble high-expression, purification and bioassay of IGFBP-3 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 398-402, 2007.
Article
em Zh
| WPRIM
| ID: wpr-328016
Biblioteca responsável:
WPRO
ABSTRACT
cDNA for Insulin-like growth factor binding protein 3 was cloned and constructed a prokaryotic expression vector--pET-DsBA-IGFBP3. The construct was transformed into E. coli BL21 (DE3)plysS. The induced fusion protein (D-IGFBP3) was expressed successfully in soluble form. We obtained D-IGFBP3 the purify of which is over 95% after purification by His affinity chromatography. The product was identified by Western-blot. The cell assay showed that the obtained fusion protein can inhibit the growth of MCF-7 and bind with IGF-I in vitro.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Ligação Proteica
/
Solubilidade
/
Proteínas Recombinantes
/
Fator de Crescimento Insulin-Like I
/
Ensaio de Imunoadsorção Enzimática
/
Expressão Gênica
/
Western Blotting
/
Cromatografia de Afinidade
/
Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Article