Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1560-1563, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-328600
Biblioteca responsável:
WPRO
ABSTRACT
The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Proteínas Recombinantes
/
Cricetulus
/
Células CHO
/
Complexo Glicoproteico GPIb-IX de Plaquetas
/
Genética
/
Mutação
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Artigo