Influence of AML1 and AML1-ETO on transcriptional regulation of nucb2 gene / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1482-1486, 2009.
Article
em Zh
| WPRIM
| ID: wpr-328616
Biblioteca responsável:
WPRO
ABSTRACT
This study was purposed to investigate the effect of AML1-ETO fusion protein resulted from hematopoietic transcription factor (AML1) and acute myeloid leukemia M(2b)(AML-M(2b)) on transcription activity of nucleobindin 2 (nucb2) promoter, and to explore the role of AML1-ETO in molecular pathogenesis of AML-M(2b). The real-time RT-PCR was used to study the regulation of AML1-ETO on nucb2 at transcription level in AML1-ETO inducible leukemia cell line, the chromatin immunoprecipitation (ChIP)-based qPCR was used to investigate the direct in vivo interaction between the AML1, AML1-ETO and nucb2 promoter in AML1-ETO positive leukemia cell line, the luciferase report gene assay was used to detect the regulation of AML1, AML1-ETO on the transcription activity of nucb2 promoter. The results showed that the expression level of nucb2 was reduced with the increase of AML1-ETO. The promoter of nucb2 could be bound by both AML1 and AML1-ETO. The promoter of nucb2 was trans-repressed by AML1 and AML1-ETO respectively. It is concluded that the nucb2 is the direct target gene of AML1 and AML1-ETO, the transcription regulation of AML1, AML1-ETO on nucb2 is carried out via repressing its promoter activity.
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Fatores de Transcrição
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Proteínas de Ligação ao Cálcio
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Regulação Leucêmica da Expressão Gênica
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Proteínas de Fusão Oncogênica
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Ativação Transcricional
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Regiões Promotoras Genéticas
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Linhagem Celular Tumoral
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Proteínas de Ligação a DNA
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Subunidade alfa 2 de Fator de Ligação ao Core
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Proteína 1 Parceira de Translocação de RUNX1
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Article