Establishment of CPP-SOM integrated cDNA microarray technology / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 422-425, 2004.
Article
em Zh
| WPRIM
| ID: wpr-328859
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To get an insight into the molecular mechanisms of diseases development and targeted therapy at the transcriptome level and search for potential therapeutic targets.</p><p><b>METHODS</b>The present researchers established a cDNA microarray platform and applied component plane presentation integrated self-organizing map (CPP-SOM) to the microarray data obtained from a differentiation model, all trans retinoic acid-induced differentiation in NB4 cells.</p><p><b>RESULTS</b>The platform included 12630 unique clones, including 9436 known genes. By CPP-SOM, the researchers were able to not only well classify the regulated genes into functionally distinct categories but also depict transcriptional changes throughout the process of the development of diseases or drug treatment.</p><p><b>CONCLUSION</b>The platform has proven to be steady and reliable, and the CPP-SOM could serve as an important and good tool for analysis of microarray data.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Análise de Sequência com Séries de Oligonucleotídeos
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Linhagem Celular Tumoral
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Métodos
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Article