Study on exons 3 and 4 of alpha-synuclein gene in Chinese familial Parkinson disease patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 536-538, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-329417
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship of Chinese familial Parkinson disease with alpha-synuclein gene.</p><p><b>METHODS</b>Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex analysis(PCR-HA) were employed to detect the abnormal mobilization in the familial Parkinson disease and sporadic Parkinson disease patients, then it was verified by gene sequencing.</p><p><b>RESULTS</b>No mutation was found in alpha-synuclein gene exons 3 and 4 by PCR-SSCP together with PCR-HA. An inserted c and an inserted t were found in intron 4, position 23 and position 67 respectively.</p><p><b>CONCLUSION</b>(1) Exons 3 and 4 of alpha-synuclein gene are not the mutational hot spots of Chinese familial Parkinson disease. (2) Two polymorphisms were found in intron 4 of alpha-synuclein gene. They are 23 ins c and 67 ins t.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doença de Parkinson
/
Reação em Cadeia da Polimerase
/
Éxons
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Polimorfismo Conformacional de Fita Simples
/
Análise Heteroduplex
/
Sinucleínas
/
Alfa-Sinucleína
/
Genética
/
Mutação
/
Proteínas do Tecido Nervoso
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2003
Tipo de documento:
Artigo