A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 510-512, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-329963
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.</p><p><b>METHODS</b>DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.</p><p><b>RESULTS</b>The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.</p><p><b>CONCLUSION</b>The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Ativação do Canal Iônico
/
Separação Celular
/
Ratos Sprague-Dawley
/
Técnicas de Patch-Clamp
/
Biologia Celular
/
Gânglios Espinais
/
Canais Iônicos
/
Métodos
/
Neurônios
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2011
Tipo de documento:
Artigo