Effect of L-tyrosine on 3beta-HSD activity of rat luteal cells in vitro / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 190-193, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-330148
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the effects of L-tyrosine on 3beta-HSD activity of rat luteal cells in vitro.</p><p><b>METHODS</b>Luteal cells were isolated from ovary tissues of female rats pretreated with PMSG and hCG. Luteal cells were cultured with 95% oxygen and 5% carbon dioxide in 37 degrees C. 3beta-HSD activity was measured by radioimmunoassay (RIA).</p><p><b>RESULTS</b>(1) 0.2 mmol x L(-1) and 2.0 mmol x L(-1) L-tyrosine significantly inhibited 3beta-HSD activity. (2) 0.2 mmol x L(-1) L-tyrosine exerted different effects on 3beta-HSD activity at different concentrations of pregnenolone (Ph). It increased 3beta-HSD activity at 0.1 micromol x L(-1) and 1 micromol x L(-1) of Pn concentration. With further increase in the concentration of Pn to 100 micromol x L(-1), the stimulating effect of L-tyrosine was switched to suppression effect. (3) L-tyrosine and L-tyrosine hydrazide both inhibited 3beta-HSD activity induced by hCG.</p><p><b>CONCLUSION</b>L-tyrosine affects 3beta-HSD activity of rat luteal cells in vitro. L-tyrosine and tyrosine hydrazide inhibits hCG induced 3beta-HSD activity.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Tirosina
/
Células Cultivadas
/
Ratos Wistar
/
3-Hidroxiesteroide Desidrogenases
/
Células Lúteas
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2004
Tipo de documento:
Artigo