Identification of cattail pollen (puhuang), pine pollen (songhuafen) and its adulterants by ITS2 sequence / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2189-2193, 2014.
Article
em Zh
| WPRIM
| ID: wpr-330323
Biblioteca responsável:
WPRO
ABSTRACT
DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study, a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced, and new primers for cattail pollen were designed according to the sequence information. The results from the NJ trees showed that the species of pine pollen, Puhuang and their adulterants can be classified as obvious monophyly. Therefore, we propose to adapt DNA barcoding methodology to accurately distinguish cattail pollen, pine pollen and their adulterant materials. It is a great help for drug regulatory agency to supervise the quality of medicinal materials.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Pólen
/
Controle de Qualidade
/
Medicamentos de Ervas Chinesas
/
Dados de Sequência Molecular
/
Química
/
China
/
Contaminação de Medicamentos
/
Classificação
/
DNA de Plantas
Tipo de estudo:
Diagnostic_studies
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Article