Application of multiplex nested RT-PCR for fast detection of PDGFRα fusion gene in myeloproliferative neoplasms / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1447-1449, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-331057
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to explore the applicable value of multiplex nested reverse transcription-polymerase chain reaction (multiplex nested RT-PCR)for the detection of platelet-derived growth factor receptor alpha (PDGFRα) fusion gene in myeloproliferative neoplasms (MPN). Bone marrow or peripheral blood samples from 146 patients with MPN were analyzed by using a novel multiplex nested RT-PCR. The result showed that PDGFRα fusion gene was found in 6 out of the 146 bone marrow or peripheral blood samples, the positive rate was 4.11%, 4 from the 6 patients received treatment with imatinib and showed therapeutic effect. It is concluded that the multiplex nested RT-PCR has a series of advantages such as high sensitivity, specificity, and time-saving, and can be applied for determination of the molecular type of MPN, and also for the diagnosis and therapy of MPN.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias da Medula Óssea
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Receptor alfa de Fator de Crescimento Derivado de Plaquetas
/
Diagnóstico
/
Fusão Gênica
/
Reação em Cadeia da Polimerase Multiplex
/
Genética
/
Métodos
/
Transtornos Mieloproliferativos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo