Application of reverse transcription-multiplex nested PCR to detect PDGFRB gene rearrangement in myeloproliferative disorders / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1443-1446, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-331058
Biblioteca responsável:
WPRO
ABSTRACT
In order to explore the value of reverse transcription(RT)-multiplex nested PCR for detecting PDGFRB gene rearrangement in myeloproliferative disorders (MPD), the PDGFRB rearrangement was detected qualitatively in 146 MPD cases by reverse transcription multiplex nested PCR. The results showed that 8 cases with PDGFRB fusion gene were found in 146 cases, the positive rate was 5.5%. Out of 8 cases with PDGFRB fusion gene, TEL-PDGRB fusion gene was found in 3 cases; HIP1-PDGFRB fusion gene in 2 cases; GIT2-PDGFRB, TP53BP1-PDGFRB and WDP48-PDGFRB fusion gene in 1 case, respectively. It is concluded that RT-multiplex nested PCR is a powerful tool for the detection of PDGFRB rearrangement, which helps to tentatively diagnose MPD and to provide the clues for targeting therapy.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rearranjo Gênico
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Receptor beta de Fator de Crescimento Derivado de Plaquetas
/
Diagnóstico
/
Reação em Cadeia da Polimerase Multiplex
/
Genética
/
Métodos
/
Transtornos Mieloproliferativos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo