Effects of bushen huoxue recipe on proliferation, differentiation and mineralization of osteoblasts in rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 1112-1114, 2005.
Article
em Zh
| WPRIM
| ID: wpr-331775
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of bushen huoxue recipe (BHR) on cell proliferation, differentiation, maturation and mineralization in osteoblasts of rats cultured in vitro.</p><p><b>METHODS</b>Osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP and ARS were used to observe the proliferation, activity of alkaline phosphatase (ALP) and the formation of mineral node of cultured osteoblasts effected by different concentrations of BHR at different time points (24, 48, 72 hrs after medication).</p><p><b>RESULTS</b>Different concentrations of BHR could enhance the cell proliferation rate and ALP activity, the effects of BHR in high and moderate concentration were more significant in enhancing the cell proliferation rate (P < 0.01). And the number of mineral node of cultured osteoblasts treated by high and moderate concentration BHR was much more than that of the untreated osteoblasts (P < 0.01).</p><p><b>CONCLUSION</b>BHR could promote the proliferation, differentiation, and mineralization of the osteoblasts.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Osteoblastos
/
Farmacologia
/
Calcificação Fisiológica
/
Medicamentos de Ervas Chinesas
/
Diferenciação Celular
/
Células Cultivadas
/
Cálcio
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Proliferação de Células
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2005
Tipo de documento:
Article