Antibody detection and sequencing analysis of hepatitis E virus in human population, swine and chicken in Sichuan region in China / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 468-471, 2008.
Article
em Zh
| WPRIM
| ID: wpr-332464
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the seroprevalence of HEV infection and genotype.</p><p><b>METHODS</b>ELISA were used for detecting anti-HEV IgG of the serum samples, the nested reverse transcriptase PCR (RT-nPCR) was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Sichuan province. The primers used for genotyping were the ORF2 region of HEV genome.</p><p><b>RESULTS</b>The anti-HEV IgG was detected positive in childrens 6.10% (41/672), adults 42.26% (280/ 661), swines 88.89% (32/36), chickens negative (0/59). 1 case of 15 serum samples of anti-HEV IgM positive and 3 of 54 swine bile samples were positive for HEV RNA by RT-PCR.Sequence analysis of 4 isolates has 92.1% to 98.6% nucleotide sequence homology. These isolates from human and swine were identified closely related to Ch-T21 strain 90.1%-96.9% sequence homology, which belonged to HEV genotype 4B.</p><p><b>CONCLUSIONS</b>The swine were the risk factors in the spread of hepatitis E virus.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Suínos
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Ensaio de Imunoadsorção Enzimática
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Anticorpos Anti-Hepatite
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Estudos Soroepidemiológicos
/
Galinhas
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China
/
Epidemiologia
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Classificação
/
Vírus da Hepatite E
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Article