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Discovery and verification of matrix gla protein, a TNM staging and prognosis-related biomarker for gastric cancer / 中华病理学杂志
Chinese Journal of Pathology ; (12): 436-441, 2010.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-333225
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze microarray datasets deposited in the public database and to identify TNM associated genes in gastric cancers.</p><p><b>METHODS</b>Microarray datasets of gastric cancer were selected from GEO database. Differentially expressed genes related to TNM staging were evaluated by significant analysis of the microarray using MultiExperiment Viewer (MEV) platform. Candidate gene expressions in gastric cancer tissues and cell lines were verified by reverse transcriptase polymerase chain reaction (RT-PCR), quantitative RT-PCR, Western blot and immunohistochemistry.</p><p><b>RESULTS</b>GES4007 dataset was re-analyzed leading to the identification of 14 genes associated with TNM staging. Over-expression of matrix gla protein (MGP) was confirmed in gastric cancer cell lines and tumor tissues by quantitative RT-PCR, Western blot and immunohistochemistry. Increased MGP expression was found in 22 of 54 cases of (40.7%) gastric cancer specimens compared to the normal gastric tissues. The up-regulation of MGP mRNA expression closely correlated with TNM stage (P = 0.001) and prognosis (P = 0.006).</p><p><b>CONCLUSIONS</b>Public databases of microarray studies are the valuable resources for data mining. MGP has been identified and confirmed as a novel biomarker for the TNM stage and prognosis of gastric cancer.</p>
Assuntos
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Neoplasias Gástricas / Proteínas de Ligação ao Cálcio / RNA Mensageiro / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Regulação para Cima / Taxa de Sobrevida / Seguimentos / Proteínas da Matriz Extracelular Tipo de estudo: Estudo observacional / Estudo prognóstico Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Pathology Ano de publicação: 2010 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Neoplasias Gástricas / Proteínas de Ligação ao Cálcio / RNA Mensageiro / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Regulação para Cima / Taxa de Sobrevida / Seguimentos / Proteínas da Matriz Extracelular Tipo de estudo: Estudo observacional / Estudo prognóstico Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Pathology Ano de publicação: 2010 Tipo de documento: Artigo
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