In-vitro amplification of oval cells with preservation of stem cell phenotype / 中华病理学杂志
Chinese Journal of Pathology
; (12): 548-552, 2010.
Article
em Zh
| WPRIM
| ID: wpr-333255
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore cell culture techniques for amplification of oval cells with preservation simultaneously of the stem cell characteristics.</p><p><b>METHODS</b>Oval cell line OC3 was cultured in RPMI 1640 supplemented with 15% fetal bovine serum and 20 µg/L EGF. Cells were harvested every 5 passages and were examined with biomarkers including OV-6, c-kit, gamma-glutamyl transpeptidase, placental form of glutathione-S-transferase (GST-P), pyruvate kinase M₂, pyruvate kinase L and albumin using techniques including RT-PCR, immunocytochemistry, and enzymo-cytochemistry.</p><p><b>RESULTS</b>OC3 cell lines could be amplified abundantly in-vitro associating with expression of infant liver cell markers at various level, including OV-6, c-kit, gamma-glutamyl transpeptidase, GST-P, pyruvate kinase M₂, but no expression of mature hepatocyte markers detected including pyruvate kinase L and albumin.</p><p><b>CONCLUSIONS</b>Amplification of OC3 cells with preservation of the stem cell phenotype and high proliferation index can be achieved up to the 79(th) passages by culturing in RPMI 1640 supplemented with 15% fetal bovine serum and 20 µg/L EGF.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fenótipo
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Piruvato Quinase
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Células-Tronco
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Antígenos de Diferenciação
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Diferenciação Celular
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Linhagem Celular
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Ratos Sprague-Dawley
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Proteínas Proto-Oncogênicas c-kit
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Técnicas de Cultura de Células
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Meios de Cultura
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2010
Tipo de documento:
Article