Chronobiologic contrast of expression of p53, p16, and cyclin D1 in hepatocarcinoma cells / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To contrast the feature of different periods of p53, p16, and cyclin D1 expression in cells of hepatocarcinoma.</p><p><b>METHODS</b>Sixty hepatocarcinoma specimens were collected and divided into three periods in one yearly cycle with each period of 20 cases. Hepatocellular carcinoma was documented in all cases by pathomorphological classification. Edmondson-Steinet grading belonged to II~III stages. The expression of gene proteins of p53, p16, and cyclin D1 was determined by immunohistochemical assay (S-P method). The expressive intensity was analysed by the rank test.</p><p><b>RESULTS</b>p16 expression showed significant difference of period (H=10.334, P<0. 05). Arpil-July was the high expressive period.</p><p><b>CONCLUSIONS</b>p16 may take an important role in the chronobiologic mechanism of gene control of hepatocyte canceration and hepatocarcinoma cell growth.</p>