HPV caused pathological changes in genital system of mice / 病毒学报
Chinese Journal of Virology
; (6): 35-40, 2009.
Article
em Zh
| WPRIM
| ID: wpr-334766
Biblioteca responsável:
WPRO
ABSTRACT
The recombined adenovirus DNA was transfected into 293 cells for packing and amplification of replication-deficient Ad-CMV-E6/E7, Ad-K14 -E6/E7 virus was purified by CsCl density gradient centrifugation , recombined adenovirus Ad-CMV-E6/E7, Ad-K14 -E6/E7 were used as experimental group, while pAd-CMV and pAdtrack-K14 were used as control group. Four of them were injected through one main vein of nude mice tail respectively. These mice were then treated with 0.05 mg 17beta-estradiol over 12 weeks. Mice were anaesthesiaed with 2.5% Avertint and the vagina, mammary gland, ovaries and uterus were dissected and fixed in 3.75% paraformaldehyde overnight at 4 degrees C. Paraffin-embedded sections, HE staining and identification of P53 and Bcl-2 protein via immunohistochemistry were performed. The expression of E6/E7 was verified by RT-PCR in different tissue of nude mice. HE staining showed evident hyperplasy in cervix-uterus transformation zone of experimental group 2. The expression of mutant P53 and Bcl-2 were higher than control group via immunohistochemical S-P method in uterus stroma-cell. Western blotting also showed that E6 protein was expressed. The expression of E6/E7 was higher than control group by human cytokeratin promoter 14 and hyperlasy changes were detected in epithelial tissue of cervix-uterus transformation zone.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ovário
/
Papillomaviridae
/
Patologia
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Fisiologia
/
Proteínas Repressoras
/
Útero
/
Vagina
/
Virologia
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Imuno-Histoquímica
/
Linhagem Celular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2009
Tipo de documento:
Article