Study on the phenotypic characteristics of Japanese encephalitis virus strains isolated from different years / 病毒学报
Chinese Journal of Virology
; (6): 427-431, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-334783
Biblioteca responsável:
WPRO
ABSTRACT
In order to reveal the phenotypic characteristics of 17 JE virus strains isolated from different years, plaque sizes, mice neurovirulence and mice neuroinvasiveness of the isolates were studied and compared. BHK21 cell monolayers were used for testing the plaque sizes. The virus neurovirulence was tested in 9-11g mice inoculated intracerebrally and the virus neuroinvasiveness was tested in 9-11g and 14-16g by subcutaneous inoculation. Results showed that all the viruses produced clear plaques on the BHK21 cell monolayers with different sizes and all the virus strains appeared high neurovirulence in the mice with higher than lg8. 0/0.03 mL virus titers, while no apparent difference among them. The neuroinvasiveness (subcutaneous virulence) tested in the 9-11g mice had shown a little difference, but when tested in the 12-14 g mice,the difference was apparent. The results demonstrated that JEV in nature were highly neurovirulent with no apparent difference. However the neuroinvasiveness of the JEV in nature was greatly different, which didn't relate to the years of isolation and genotypes, but most of the viruses isolated from patients showed higher neuroinvasiveness.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Ensaio de Placa Viral
/
Virologia
/
Virulência
/
Linhagem Celular
/
China
/
Encefalite Japonesa
/
Vírus da Encefalite Japonesa (Espécie)
/
Genética
/
Genótipo
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2008
Tipo de documento:
Artigo