Construction of rabbit hemorrhagic disease virus replicons and its replication in RK-13 cells / 病毒学报
Chinese Journal of Virology
; (6): 481-484, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-334861
Biblioteca responsável:
WPRO
ABSTRACT
To provide an efficient and safe technology platform for studying the replication and pathogenesis mechanisms of RHDV, the interaction between the RHDV and its host cells, a replicon system of RHDV, was constructed based on the infectious cDNA clone of RHDV, in which VP60 gene encoding the capsid protein was deleted, but all the necessary protease coding regions and non-coding regions were retained. Results from RT-PCR, IFA and qRT-PCR confirmed that the replicon RNA could efficiently replicate in RK-13 cells. Besides, the results also suggested that the capsid protein which is the structural protein of RHDV is necessary for maintaining the viral infectivity.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Replicon
/
RNA Viral
/
Imunofluorescência
/
Vírus da Doença Hemorrágica de Coelhos
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Proteínas do Capsídeo
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2007
Tipo de documento:
Artigo