Recombinant HEV caspid protein p239 specifically attached on HepG2 cells and blocked the infection of wild-type HEV on liver cells / 病毒学报
Chinese Journal of Virology
; (6): 331-334, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-334888
Biblioteca responsável:
WPRO
ABSTRACT
By using Western blot and immunofluorescence assays, the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature. Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells, indicating that p239 and HEV competed the same targeting site on these cells. These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ligação Proteica
/
Fatores de Tempo
/
Virologia
/
Proteínas Recombinantes
/
Western Blotting
/
Imunofluorescência
/
Vírus da Hepatite E
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Hepatócitos
/
Proteínas do Capsídeo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2007
Tipo de documento:
Artigo