Fenofibrate inhibits tumor necrosis factor-alpha-induced expression of CD40 and matrix metalloproteinase in human vascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1383-1387, 2006.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-334920
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effects of fenofibrate on TNF-alpha-induced CD40 expression and matrix metalloproteinase (MMP) activity in human vascular endothelial cells (HUVECs).</p><p><b>METHODS</b>Quantitative RT-PCR and flow cytometry were employed to evaluate the effect of fenofibrate on TNF-alpha-induced CD40 mRNA and cell surface CD40 expression in HUVECs, and gelatin zymography was used to determine the effect of fenofibrate on the gelatinolytic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HUVECs.</p><p><b>RESULTS</b>Fenofibrate at the concentrations of 5x10(-5), 1x10(-4) and 2x10(-4) mol/L significantly reduced TNF-alpha-induced increment of CD40 mRNA and cell surface CD40 expressions (P<0.01), with the maximal inhibition achieved at the concentration of 1x10(-4) mol/L. Fenofibrate at 2x10(-4) mol/L did not further decrease CD40 expression induced by TNF-alpha. Fenofibrate significantly inhibited the stimulatory effect of TNF-alpha on MMP-2 and MMP-9 activities in HUVECs.</p><p><b>CONCLUSION</b>Fenofibrate reduces TNF-alpha-induced increment of CD40 expression and MMP-2 and MMP-9 activities in HUVECs.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fenofibrato
/
Veias Umbilicais
/
RNA Mensageiro
/
Células Cultivadas
/
Fator de Necrose Tumoral alfa
/
Antígenos CD40
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Metaloproteinase 2 da Matriz
/
Metaloproteinase 9 da Matriz
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo