Expression of oxyntomodulin in bifidobacteria and effect of oxyntomodulin-transformed bifidobacteria on the body weight of obese mice / 南方医科大学学报
Journal of Southern Medical University
; (12): 1796-1798, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-336083
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of pBBADs-OXM-transformed bifidobacteria on the body weight of obese mice.</p><p><b>METHODS</b>B. longum was transformed with pBBADs-OXM by electroporation, and arabopyranose-induced oxyntomodulin expression by the bacterium was detected by ELISA. pBBADs-OXM-transformed bifidobacteria was administered orally obese mice on a daily basis with pBBADs-GFP-transformed bifidobacteria as the negative control, and the body weight changes of the mice were observed.</p><p><b>RESULTS</b>OXM was detected by ELISA not only in the supernatant but also the precipitant of the transformed bacterial culture. The body weight of the obese mice fed with pBBADs-OXM-transformed bifidobacteria decreased significantly compared with that of the mice in the obese model group (P<0.05).</p><p><b>CONCLUSION</b>Administration of pBBADs-OXM-transformed B.longum can reduce the body weight of obese mice.</p>
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Depressores do Apetite
/
Bifidobacterium
/
Peso Corporal
/
Proteínas Recombinantes
/
Distribuição Aleatória
/
Administração Oral
/
Eletroporação
/
Tratamento Farmacológico
/
Escherichia coli
/
Oxintomodulina
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo