Expression of porcine interferon-gamma and its safe antiviral assay / 生物工程学报
Chinese Journal of Biotechnology
; (12): 439-447, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-336208
Biblioteca responsável:
WPRO
ABSTRACT
In order to ensure the biosafety of the IFN-gamma antiviral activity assay, we used a replication-deficient VSV carrying GFP as an interferon sensitive indicator virus (VSVdeltaG*G). The antiviral activities of porcine IFN-gamma expressed in Escherichia coli and in baculovirus on MDBK cells were assessed. The results showed that the antiviral activity of porcine IFN-gamma expressed in baculovirus could reach 10(5) IU/mL, while the porcine IFN-gamma expressed in E. coli showed some antiviral activity (32 IU/mL) after refolding. The results of the VSVdeltaG*G-based antiviral assay were almost identical to that of the VSV*GFP-based assay, suggesting it is highly feasible to use VSVdeltaG*G as a substitute for VSV*GFP, making assays for IFN-gamma antiviral activity safer and more accurate.
Texto completo:
Disponível
Contexto em Saúde:
ODS3- Meta 3D Reforçar a capacidade de alerta precoce, redução e gestão de riscos de saúde nacionais e globais
/
Doenças Negligenciadas
Problema de saúde:
Riscos Biológicos
/
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antivirais
/
Farmacologia
/
Fisiologia
/
Suínos
/
Proteínas Recombinantes
/
Baculoviridae
/
Interferon gama
/
Vesiculovirus
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Proteínas de Fluorescência Verde
/
Escherichia coli
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Artigo