Expression and purification of fusion protein CTP-SOD in Pichia pastoris and antioxidant capacity analysis / 生物工程学报
Chinese Journal of Biotechnology
; (12): 324-329, 2010.
Article
em Zh
| WPRIM
| ID: wpr-336224
Biblioteca responsável:
WPRO
ABSTRACT
Cytoplasmic transduction peptide (CTP) is a newly designed transduction peptide, by which special molecules can be carried out and localized into cytoplasmic compartment. Superoxide dismutase (SOD) is a protein that is difficult to go into cytoplasm. In this study, CTP-SOD fusion gene was amplified from human cDNA by PCR, and the active recombinant protein was successfully expressed in Pichia pastoris. HeLa cells pretreated with CTP-SOD showed a significantly improved survival against the pyrogallol-induced oxidative stress, suggesting CTP-SOD could cross the cell membrane more efficiently and protect cells from oxidative stress.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
/
Pichia
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Superóxido Dismutase
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Proteínas Recombinantes de Fusão
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Células HeLa
/
Transdução de Sinais
/
Estresse Oxidativo
/
Citoplasma
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Peptídeos e Proteínas de Sinalização Intracelular
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Article