Transplanted bone marrow stromal cells improve cognitive dysfunction due to aging hypoperfusion in rats / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 3620-3625, 2010.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-336573
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Aging is an important risk factor for vascular dementia, and D-galactose (D-gal) injection can simulate the pathology of aging. Two-vessel occlusion of common carotid arteries (2VO) is the most popular model for vascular dementia. This study was aimed to investigate the possibility of D-gal injection plus 2VO simulating cognitive impairment of aging vascular dementia; and whether transplanted bone marrow stromal cells (BMSCs) can improve the cognitive function induced by D-gal injection plus 2VO.</p><p><b>METHODS</b>Totally 30 male Sprague-Dawley rats were divided into 5 groups equivalently control group, D-gal group, D-gal + 2VO group, D-gal + 2VO + saline water group, and D-gal + 2VO + BMSCs group. Aging hypoperfusion rats were created by subcutaneous injection of D-gal and occlusion of two common carotid arteries. BMSCs or saline water was stereotactically transplanted into the subventricular zone as treatment vehicles at 24 hours post operation. Two-way repeat analysis of variance (ANOVA) was used for significance analysis of 5 groups at 6 weeks post transplantation; moreover, Tamhane's test (equal variance not assumed) and least significant difference (LSD) test (equal variance assumed) were used for pairwise comparison in Morris water maze (MWM).</p><p><b>RESULTS</b>Transplanted BMSCs distributed around the lateral ventricles and acquired the phenotypes of neurons and astrocytes. In terms of swimming path distance and escape latency in MWM, D-gal + 2VO + BMSC group showed significant improvement than the D-gal + 2VO group but was still obviously worse than the control group (both P < 0.05). There was no significant difference in swimming speed for all 5 groups.</p><p><b>CONCLUSIONS</b>D-gal plus 2VO induces cognitive dysfunction. The engrafted BMSCs exhibit the beneficial effect on cognitive function via promotion interactively with host brain.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Psicologia
/
Cirurgia Geral
/
Transplante
/
Envelhecimento
/
Células da Medula Óssea
/
Demência Vascular
/
Transplante de Medula Óssea
/
Estenose das Carótidas
/
Células Estromais
/
Ratos Sprague-Dawley
Tipo de estudo:
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2010
Tipo de documento:
Artigo