Overexpression of FKS1 to improve yeast autolysis-stress / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1344-1354, 2015.
Article
em Zh
| WPRIM
| ID: wpr-337486
Biblioteca responsável:
WPRO
ABSTRACT
With the development of high gravity brewing, yeast cells are exposed to multiple brewing-associated stresses, such as increased osmotic pressure, enhanced alcohol concentration and nutritional imbalance. These will speed up yeast autolysis, which seriously influence beer flavor and quality. To increase yeast anti-autolytic ability, FKS1 overexpression strain was constructed by 18S rDNA. The concentration of β-1,3-glucan of overexpression strain was 62% higher than that of wild type strain. Meantime, FKS1 overexpression strain increased anti-stress ability at 8% ethanol, 0.4 mol/L NaCl and starvation stress. Under simulated autolysis, FKS1 showed good anti-autolytic ability by slower autolysis. These results confirms the potential of FKS1 overexpression to tackle yeast autolysis in high-gravity brewing.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Saccharomyces cerevisiae
/
Autólise
/
Cerveja
/
Hipergravidade
/
Biologia Celular
/
Proteínas de Saccharomyces cerevisiae
/
Equinocandinas
/
Genética
/
Glucosiltransferases
/
Proteínas de Membrana
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2015
Tipo de documento:
Article