Association of genetic polymorphism of XPD with chromosomal damage in workers exposed to radiation / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 718-721, 2007.
Article
em Zh
| WPRIM
| ID: wpr-338932
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore association genetic polymorphism of XPD with chromosomal damage in workers exposed to radiation.</p><p><b>METHODS</b>182 workers exposed to radiation for at least one year with chromosomal damage were selected as cases based on a general health examination for all workers exposed to radiation in Tangshan city. The control group without chromosomal damage was matched to case by age (within 5 years), sex, work unit, type of exposed to radiation, cumulate serve length (within 1 year) according to 1:1. The micro whole blood cultivation was used for the chromosome analysis. The chromosome aberration type and rate were observed and counted. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to examine the genotype of three XPD loci (751, 312 and 156).</p><p><b>RESULTS</b>The frequency of XPD 751 AA in cases was higher than that in controls (P < 0.05). The frequency of 751 allele in case group was statistically higher than that in the control groups (P < 0.05). No statistical difference was found in the frequencies of XPD 312 genotype and allele between the case and control group (P > 0.05). 156 mutant gene type in case group was higher than that in the control groups. The frequency of 156 A allele in case group were higher than that of the control groups (P < 0.05). The frequency of genotype with both 751AA and 156CA or 751AA and 156AA was higher in cases than that of controls (P < 0.05).</p><p><b>CONCLUSION</b>XPD 751AA genotype is a possible risk factor for radiation-induced chromosomal damage. XPD 156 mutant gene type is a possible risk factor for radiation-induced chromosomal damage. Individuals with both XPD 751AA and 156 (CA+AA) genotypes are susceptible to radiation-induced chromosomal damage. No association of XPD 312 polymorphism with radiation-induced chromosomal damage is found.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Radiação
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Efeitos da Radiação
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Polimorfismo de Fragmento de Restrição
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Estudos de Casos e Controles
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Exposição Ocupacional
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Aberrações Cromossômicas
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Predisposição Genética para Doença
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Proteína Grupo D do Xeroderma Pigmentoso
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Genética
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2007
Tipo de documento:
Article