Apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 581-585, 2007.
Article
em Zh
| WPRIM
| ID: wpr-338981
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field and to investigate the possible mechanism of human reproductive health effects caused by 50 Hz magnetic field.</p><p><b>METHODS</b>Cultured human villous trophoblasts were exposed to 50 Hz magnetic field at 0.4 mT for 6, 48, 72 hours. Gene expressions of Bcl-2, Bax, Caspase-3, p53 and Fas were analyzed using real-time reverse transcription polymerase chain reaction (RT-PCR) assay.</p><p><b>RESULTS</b>Within 72 hours, the average fold change for each gene was near 1.00, and there was no significant difference on expression pattern in each gene between exposure and control groups (P > 0.05).</p><p><b>CONCLUSION</b>0.4 mT 50 Hz magnetic field does not affect the apoptosis-related gene expression of human villous trophoblasts in vitro.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Trofoblastos
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Células Cultivadas
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Regulação da Expressão Gênica
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Proteína Supressora de Tumor p53
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Receptor fas
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Proteínas Proto-Oncogênicas c-bcl-2
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Proteína X Associada a bcl-2
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Caspase 3
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Campos Magnéticos
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Metabolismo
Limite:
Female
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Humans
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Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2007
Tipo de documento:
Article