Expression of CD45 in the serum of patients with Crohn's disease / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To search for differentially expressed proteins in the serum of patients with Crohn's disease.</p><p><b>METHODS</b>Serum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins.</p><p><b>RESULTS</b>2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn's disease compared with that in normal adults (P<0.05). The protein was identified as CD45 using mass spectrometry.</p><p><b>CONCLUSION</b>CD45 overexpression in the serum of patients with Crohn's disease may play a role in the disequilibrium of the immune system.</p>