Development of time-resolved fluoroimmunoassay kit for detection of IgM antibodies against hepatitis B core antigen / 南方医科大学学报
Journal of Southern Medical University
; (12): 84-86, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-339059
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a time-resolved fluoroimmunoassay (TRFIA) kit for clinical detection of IgM antibodies to hepatitis B core antigen (HBc).</p><p><b>METHODS</b>Immunocapture method was used to develop the TRFIA kit for detection of the anti-HBc IgM antibodies, and the precision, cross-reactivity and sensitivity of the kit were tested with the clinical serum samples.</p><p><b>RESULTS</b>The intra- and inter-assay coefficients of variation of the TRFIA kit were 4.8%-7.2% and 7.5%-8.6%, respectively, and no cross-reactivity with anti-HBs, anti-HBc-IgG or anti-HBe was found. Comparison of the results of the TRFIA kit and enzyme-linked immunosorbent assay (ELISA) demonstrated greater sensitivity of the kit than ELISA in detecting the anti-HBc IgM antibodies in 584 serum samples. According to the detection results in 300 serum samples from healthy donors, the cutoff value of the TRFIA kit was 4.5 times of the fluorescence value of the negative control.</p><p><b>CONCLUSION</b>This TRFIA kit for detecting anti-HBc IgM antibodies meets the demand for clinical application and can replace the ELISA kits.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Virologia
/
Sangue
/
Imunoglobulina M
/
Fluorimunoensaio
/
Sensibilidade e Especificidade
/
Alergia e Imunologia
/
Hepatite B
/
Anticorpos Anti-Hepatite B
/
Antígenos do Núcleo do Vírus da Hepatite B
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo