In vitro cytotoxicity of 131I-Rituximab against B-cell lymphoma cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 40-43, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-339071
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the specific cytotoxicity of (131)I-Rituximab against CD20-positive B-cell lymphoma cells.</p><p><b>METHODS</b>Rituximab was labeled with (131)I using IODO-GEN method, and the dose-effects of various concentrations of (131)I-Rituximab, (131)I alone and Rituximab in Raji cells were evaluated by MTT assay to determine the optimal dose according to the dose-effect curves. The cytotoxicity of (131)I-Rituximab, (131)I and Rituximab was assessed in CD20-positive Ramos (RA-1) cells, Raji cells and CD20-negative Molt-4 cells according to the changes of the survival rates. Giemsa staining was used to evaluate the antitumor effect of (131)I-Rituximab in Raji cells by measuring the mitosis index (MI).</p><p><b>RESULTS</b>(131)I-Rituximab presented with a dose-dependent cytotoxicity against Raji cells. At the specific activity of 60 microCi/ml, (131)I-Rituximab resulted in significantly higher growth inhibition rate of the cells than Rituximab (P<0.05). The inhibition rate of Raji cells treated with (131)I-Rituximab, (131)I, or Rituximab for 96 h were comparable with the rates in Ramos RA-1 cells, but significantly higher than the rates in Molt-4 cells. The MI values in (131)I-Rituximab group were significantly lower than those in the other groups (P<0.001).</p><p><b>CONCLUSION</b>(131)I-Rituximab can induce specific cytotoxicity against CD20-positive tumor cells, and may potentially serve as an agent for targeted radioimmunotherapy for CD20-positive B-cell lymphoma.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Células Tumorais Cultivadas
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Linfoma de Células B
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Radioimunoterapia
/
Apoptose
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Antígenos CD20
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Relação Dose-Resposta a Droga
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Alergia e Imunologia
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Anticorpos Monoclonais Murinos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo