Relationship between amyloid beta-protein and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide against oxidative stress induced damage on neuro-2a cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 171-174, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-339648
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To observe the relationship between amyloid beta-protein (Abeta) and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide (PACAP, PACAP-27) against damage induced by oxidative stress (H2O2) in neurem-2a cells.</p><p><b>METHODS</b>With cultured neuro-2a cells the cell survival and apoptosis were measured by MTT assay, Hoechest33258 staining, DNA ladder and the percentage of small DNA fragment.</p><p><b>RESULTS</b>Concentration-dependent toxicity was induced with H2O2 treatment for 24 h. The neurotoxicity of H2O2 was increased by about 10 times with cotreatment neurons with amyloid beta-protein fragment 25-35 (Abeta(25-35)). While decrease the percentage of small DNA fragmentation the cell survival was increased with co-treatment with PACAP-27(which were added to the culture everyday). The effect of PACAP was not reversed with antagonist of PACAP receptor, PACAP(6-27).</p><p><b>CONCLUSION</b>Abeta and H2O2 can promote each other's neurotoxicity. Cultured neurons were protected by PACAP27 from the neurotoxicity of H2O2 but not through the activation of PACAP-27 receptor.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Sobrevivência Celular
/
Células Cultivadas
/
Peptídeos beta-Amiloides
/
Apoptose
/
Estresse Oxidativo
/
Biologia Celular
/
Polipeptídeo Hipofisário Ativador de Adenilato Ciclase
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Toxicidade
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Peróxido de Hidrogênio
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2003
Tipo de documento:
Artigo