Analysis of full-length gene sequence of rabies vaccine virus aG strain / 病毒学报
Chinese Journal of Virology
; (6): 404-409, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-339937
Biblioteca responsável:
WPRO
ABSTRACT
To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full-length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5' leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4. 0 software. aG strain was 11 925nt(GenBank accession number JN234411) in length and belonged to the genotype I . The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus. the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.
Texto completo:
Disponível
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
/
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Objetivo 10: Doenças transmissíveis
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Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
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Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
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Raiva
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Vírus da Raiva
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Especificidade da Espécie
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Virologia
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Dados de Sequência Molecular
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Vacina Antirrábica
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Sequência de Bases
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China
/
Sequência de Aminoácidos
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Artigo