Cloning and functional research of Arp2/3-P40/ARPC1 subunit of Sf9 cells / 病毒学报
Chinese Journal of Virology
; (6): 601-608, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-339998
Biblioteca responsável:
WPRO
ABSTRACT
The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with WASP proteins that can activate Arp2/3 complex and induce the actin polymerization. In order to explore the role of Arp2/3 complex in the baculovirus replication, the P40 subunit of Arp2/3 complex from Sf9 (Spodoptera frugiperda 9) cell line was cloned and characterized. Immunofluorescent microscopy assay indicated that P40 was recruited to the inner-side of nuclear membrane during virus infection, which was in accordance with nuclear F-actin distribution in virus-infected cells as documented in our previous research, suggesting P40 could be used to track Arp2/3 complex subcellular distribution changes during virus infection. In addition, co-immunoprecipitation assay demonstrated that P40 interacted with P78/83 only in virus-infected cells, suggesting that actin polymerization induced by P78/83-Arp2/3 complex during baculovirus infection was regulated by some unidentified virus factors.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Ligação Proteica
/
Virologia
/
Dados de Sequência Molecular
/
Linhagem Celular
/
Química
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Nucleopoliedrovírus
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo