Detecting the abnormal expression of PDGFRA gene in eosinophilia by FISH / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1377-1380, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-340494
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the abnormal expression of PDGFRA gene in eosinophilia by FISH. Translocations of PDGFRA gene in 13 patients with eosinophilia were detected by using 4q12 three-color probe and FISH technology. Fifteen people were used as control to establish the normal cut-off value of fluorescence signal of PDGFRA. The results indicated that 1 out of 13 patients with eosinophilia was corrected and was diagnosed as CML. The fusion gene of FIP1L1-PDGFRA (F/P) was found in 2 patients and the positive rate of F/P fusion gene detected by probe 4q12 was 17% in the 12 patients with eosinophilia. Other translocation forms involving PDGFRA gene were not found. It is concluded that a variety of translocation forms of PDGFRA gene can be detected in patients with eosinophilia by using 4q12 three-color probe and FISH technology, which can provide important information for assessing diagnosis and treatment.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Translocação Genética
/
Cromossomos Humanos Par 4
/
Proteínas de Fusão Oncogênica
/
Hibridização in Situ Fluorescente
/
Receptor alfa de Fator de Crescimento Derivado de Plaquetas
/
Eosinofilia
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2014
Tipo de documento:
Artigo