Stability study in biological samples and metabolites analysis of astragaloside IV in rat intestinal bacteria in vitro / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4258-4264, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-341873
Biblioteca responsável:
WPRO
ABSTRACT
To figure out the stability and intestinal bacteria metabolites of rats in vitro of astragaloside IV ( AST), this research was done to explore the stability of AST in the artificial gastric juice. artificial intestinal juice and rat liver homogenate and the metabolism in rat intestinal in vitro. HPLC was used to calculate the remaining rate of AST in biological samples by measuring the content of AST, while metabolites were determined by combining the methods of TLC, HPLC and LC-MS/MS. It turned out that AST was difficult to metabolize in the artificial gastric juice, artificial intestinal juice and rat liver. Also, the metabolic pathway of AST was stepped by deglycosylation. Firstly, AST was converted to its secondary etabolites (6-O-β-D-glucopyranosyl- cycloastragenol, CMG) by removal of xylose moiety at C-3, then transformed into cycloastragenol (CAG) after hydrolytic removal of the glucose moiety at C-6. All the results suggested that the metabolism of AST in vivo occurs mainly in the intestinal by hydrolysis of glycosyl. In conclusion, hydrolysis of intestinal flora is the main reason that AST metabolizes.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Saponinas
/
Bactérias
/
Triterpenos
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Ratos Sprague-Dawley
/
Estabilidade de Medicamentos
/
Espectrometria de Massas em Tandem
/
Intestinos
/
Fígado
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo