Sequence detection of HBV-DNA P and C region in HIV/HBV superinfection subjects with drug resistance to HAART / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 507-509, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-341965
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To further study the resistance of HBV to high activity antiretrovirus therapy(HAART).</p><p><b>METHODS</b>HBV-DNA was quantitatively detected by real-time PCR in 36 HIV/HBV superinfection subjects, C region and P region HBV-DNA in high copies HBV-DNA subjects were detected by routine PCR, PCR products were purified and sequenced and compared with the HBV international Genbank using BLSAT softy ware.</p><p><b>RESULT</b>HBV-DNA was positive in 4 of 36 patients (11.1%) and another 3 had low copies(<10(4)copies/ml), one had a high HBV-DNA copies (10(7)copies/ml). It's HBV-DNA C region sequence had mutation on 2 sites (nt 2412 T/C; nt 2413 T/C) and 1 mutation P region (nt 741 A/G, also YMDD/YVDD) compared with HBV international Genbak reference sequence.</p><p><b>CONCLUSION</b>The HBV resistance to HAART may be related with multiple genetic mutations in the C and P regain of HBV-DNA.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
DNA Viral
/
Sequência de Bases
/
Infecções por HIV
/
Química
/
Vírus da Hepatite B
/
Terapia Antirretroviral de Alta Atividade
/
Farmacorresistência Viral
/
Tratamento Farmacológico
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2003
Tipo de documento:
Artigo