Over-expressed MiR-103 promotes porcine adipocyte differentiation / 生物工程学报
Chinese Journal of Biotechnology
; (12): 927-936, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-342428
Biblioteca responsável:
WPRO
ABSTRACT
To clarify the function of miR-103 in the differentiation of porcine preadipocyte, we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker--PPARgamma and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARgamma, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
RNA Mensageiro
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Transfecção
/
Diferenciação Celular
/
Adenoviridae
/
Adipócitos
/
Biologia Celular
/
MicroRNAs
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2012
Tipo de documento:
Artigo