Construction and identification of plasmid vector encoding two survivin shRNA / 中华男科学杂志
National Journal of Andrology
; (12): 512-515, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-343585
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct and identify the eukaryotic expression plasmids encoding two short hairpin RNA (shRNA) of survivin for the purpose of paving the way for the studies of targeted gene therapy for prostatic carcinoma (PCa).</p><p><b>METHODS</b>Two shRNA of survivin were designed and synthesized respectively, and then both were cloned into plasmids. Finally, the recombinant plasmids were confirmed by sequencing and agarose gel electrophoresis after restriction digestion.</p><p><b>RESULTS</b>The recombinant plasmids encoding two survivin shRNA were constructed and the aim sequence obtained.</p><p><b>CONCLUSION</b>Successful construction of the recombinant provides a sound basis for the research of targeted gene therapy for PCa.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Transfecção
/
Clonagem Molecular
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Proteínas de Fluorescência Verde
/
Proteínas Inibidoras de Apoptose
/
Vetores Genéticos
/
Genética
/
Proteínas Associadas aos Microtúbulos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo