Development of radioimmunoassay for human sperm protein 17 / 中华男科学杂志
National Journal of Andrology
; (12): 510-515, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-343586
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method of radioimmunoassay for human sperm protein 17(Sp17) determination.</p><p><b>METHODS</b>Anti-recombinant human Sp17 antibody was prepared, the labeling of 125I-rhSp17 performed by chloramine T method, and radioimmunoassay of Sp17 developed.</p><p><b>RESULTS</b>The assay range was 3.3 to approximately 800 microg/L, the sensitivity was 2.0 microg/L, and the intra- and inter-assay coefficients of variation (CV) were 7.5% to approximately 9.8% and 8.2% to approximately 13.2%, respectively. The serum Sp17 level in normal subjects was (15.60 +/- 7.66) microg/L (n = 59).</p><p><b>CONCLUSION</b>This radioimmunoassay of Sp17 fulfills the reasonable requirements of clinical routine and scientific studies in terms of specificity, sensitivity and practicability. Measurement of Sp17 concentration is useful for assessing its native distribution and aberrant expression.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Proteínas Recombinantes
/
Radioimunoensaio
/
Proteínas de Transporte
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Alergia e Imunologia
/
Métodos
/
Antígenos de Superfície
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo