Effect of tensile stress on human heel skin fibroblast proliferation in vitro / 中国骨伤
China Journal of Orthopaedics and Traumatology
; (12): 838-842, 2014.
Article
em Zh
| WPRIM
| ID: wpr-345298
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of tensile stress on human heel skin fibroblast proliferation in vitro, providing a theoretical basis for preventing the wound edge skin necrosis and nonunion after calcaneal fracture surgery.</p><p><b>METHODS</b>Fibroblast cells were taken from lateral heel skin of a 40 year-old-man, then cultured and subcultured in vitro. After that, they were divided into three groups: 0 hours group, 6 hours group and 24 hours group and were tested by tensile stress testing. The levels of TGF-β1 and IL-6 in nutrient fluid were measured. Transmission electron microscope and light microscope was applied for observe mitochondria and nucleus.</p><p><b>RESULTS</b>Under 10% of the tensile stress, mitochondria decreased, the levels of TGF-β1 and IL-6 in nutrient fluid were decreased and cell proliferation was inhibited gradually with time increasing.</p><p><b>CONCLUSION</b>The human lateral heel skin in a long-time tensile stress state is an important cause of wound edge skin necrosis and nonunion after calcaneus fracture surgery.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fisiologia
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Pele
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Resistência à Tração
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Técnicas In Vitro
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Calcanhar
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Células Cultivadas
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Química
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Interleucina-6
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Biologia Celular
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Proliferação de Células
Limite:
Adult
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Humans
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Male
Idioma:
Zh
Revista:
China Journal of Orthopaedics and Traumatology
Ano de publicação:
2014
Tipo de documento:
Article