The roles of Smad 2/3 in transforming growth factor-beta 1 signaling in human dental pulp cells / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 39-42, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-347310
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the roles of Smad 2/3 in transforming growth factor-beta(1) (TGF-beta(1)) signaling by human dental pulp cells.</p><p><b>METHODS</b>Laser scanning confocal microscope was used to observe translocation of Smad 2/3 from plasma into nucleus in cultured dental pulp cells at early stage of TGF-beta(1) treatment, and changes of Smad 2/3 protein expression at later stage were evaluated by Western blot analyses.</p><p><b>RESULTS</b>The expression of Smad 2/3 (fluorescence intensity) kept decreasing in cytoplasm but increasing in nucleus within 2 h after TGF-beta(1) treatment, forming a trend that Smad 2/3 translocated into nucleus from cytoplasma. The total amount of Smad 2 protein remained unchanged before and after TGF-beta(1) treatment, but the expression level of Smad 3 decreased markedly after 24 h treatment and kept dropping by 48 h.</p><p><b>CONCLUSIONS</b>The results suggest that the Smad 2/3 may be the downstream signal transducers of TGF-beta(1) in human dental pulp cells and Smad 2/3 may mediate TGF-beta(1) signaling by translocation early in TGF-beta(1) treatment, while down-regulation of Smad 3 expression by TGF-beta(1) at later stage is involved in negative modulation of TGF-beta(1) signaling.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Fatores de Tempo
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Transporte Biológico
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Transdução de Sinais
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Transativadores
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Núcleo Celular
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Células Cultivadas
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Western Blotting
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Fator de Crescimento Transformador beta
Limite:
Adolescente
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Adulto
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Criança
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Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2003
Tipo de documento:
Artigo