Induction of apoptosis by proteasome inhibitor MG-132 in human erythroleukemia cell line K562 / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 255-258, 2009.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-347943
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether proteasome inhibitor MG-132 induces apoptosis of human erythroleukemia cell line K562 and possible mechanisms.</p><p><b>METHODS</b>K562 cells were incubated with RPMI 1640 and exposed to 0, 1, 5, 10, 15 micromol/L of MG-132 for 24 hrs, respectively. The apoptosis of cells were detected by fluorescence microscope, DNA fragments and flow cytometry. The NF-kappaB mRNA expression was quantified by reverse transcription-polymerase chain reaction (RT-PCR). Expression of NF-kappaB and caspase-3 was semiquantitatively analyzed with SABC techniques. Caspase-3 activities were measured with a colorimetric method.</p><p><b>RESULTS</b>The growth of K562 cells was inhibited and the apoptosis of the cells increased after MG-132 treatment in a dose-dependent manner. After 24 hrs of 15 micromol/L MG-132 treatment, the percentage of apoptotic cells (26.5+/-0.6%) increased significantly when compared with the untreated controls (1.2+/-0.1%) (P<0.01). MG-132 treatment decreased the mRNA and protein expression of NF-kappaB, and increased the protein expression of caspase-3.</p><p><b>CONCLUSIONS</b>MG-132 can induce apoptosis of human erythroleukemia cell line K562 through the down-regulation of NF-kappaB expression and up-regulation of caspase-3 expression.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Inibidores de Cisteína Proteinase
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NF-kappa B
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Apoptose
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Células K562
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Relação Dose-Resposta a Droga
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Caspase 3
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Inibidores de Proteassoma
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Leupeptinas
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2009
Tipo de documento:
Artigo