Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 412-414, 2007.
Article
em Zh
| WPRIM
| ID: wpr-348034
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.</p><p><b>METHODS</b>Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.</p><p><b>RESULTS</b>At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.</p><p><b>CONCLUSION</b>The Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.</p>
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Base de dados:
WPRIM
Assunto principal:
Osteoclastos
/
Estresse Mecânico
/
Xantenos
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Medula Óssea
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Células da Medula Óssea
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Células Cultivadas
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Cálcio
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Compostos de Anilina
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Article